rabbit anti-per2 primary antibody per21-a Search Results


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Bio-Techne corporation bmal1 antibody
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Bio-Techne corporation luciferase antibody (luci 21 1-107) - bsa free
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St Johns Laboratory per2
CNOT1 regulates the stability of <t>Per2</t> through regulating poly (A) length.
Per2, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GeneTex anti-p84 gtx70220
CNOT1 regulates the stability of <t>Per2</t> through regulating poly (A) length.
Anti P84 Gtx70220, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA mouse anti-neun
CNOT1 regulates the stability of <t>Per2</t> through regulating poly (A) length.
Mouse Anti Neun, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fortis Life Sciences anti clock
CNOT1 regulates the stability of <t>Per2</t> through regulating poly (A) length.
Anti Clock, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA anti-glua1 phospho-ser845 antibody
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
Anti Glua1 Phospho Ser845 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA rabbit polyclonal anti-c-fos abe457
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
Rabbit Polyclonal Anti C Fos Abe457, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bethyl a302 614a
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
A302 614a, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MBL Life science anti–α-tubulin
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
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GeneTex anti-per1
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
Anti Per1, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abnova anti-hsd3b1
a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of <t>GluA1</t> S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.
Anti Hsd3b1, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CNOT1 regulates the stability of Per2 through regulating poly (A) length.

Journal: RNA Biology

Article Title: CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner

doi: 10.1080/15476286.2022.2071026

Figure Lengend Snippet: CNOT1 regulates the stability of Per2 through regulating poly (A) length.

Article Snippet: Antibodies against the following were used: Cnot1, Cnot3, Cnot6, Cnot6l, Cnot7, and Cnot8 (mouse monoclonal antibodies; generated by Bio Matrix Research and Research Center for Advanced Science and Technology, The University of Tokyo), BRF1/2 (#2119; Cell Signalling Technology), KSRP (A302-021A; Bethyl Laboratories), α-tubulin (#T9026; Sigma), PER2 (PER21-A, Alpha diagnostic) for mouse tissue, PER2 (STJ115134, St. John’s Laboratory) for HEK293T cells, BMAL1 (A302-616A; Bethyl Laboratories), 4E-BP1 (#9644S, Cell Signalling Technology) and GAPDH (#2118; Cell Signalling Technology).

Techniques:

BRF1 binds to Per2 and regulates its stability.

Journal: RNA Biology

Article Title: CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner

doi: 10.1080/15476286.2022.2071026

Figure Lengend Snippet: BRF1 binds to Per2 and regulates its stability.

Article Snippet: Antibodies against the following were used: Cnot1, Cnot3, Cnot6, Cnot6l, Cnot7, and Cnot8 (mouse monoclonal antibodies; generated by Bio Matrix Research and Research Center for Advanced Science and Technology, The University of Tokyo), BRF1/2 (#2119; Cell Signalling Technology), KSRP (A302-021A; Bethyl Laboratories), α-tubulin (#T9026; Sigma), PER2 (PER21-A, Alpha diagnostic) for mouse tissue, PER2 (STJ115134, St. John’s Laboratory) for HEK293T cells, BMAL1 (A302-616A; Bethyl Laboratories), 4E-BP1 (#9644S, Cell Signalling Technology) and GAPDH (#2118; Cell Signalling Technology).

Techniques:

Proposed model of CCR4-NOT/BRF1 mediated decay of Per2 mRNA.

Journal: RNA Biology

Article Title: CNOT1 regulates circadian behaviour through Per2 mRNA decay in a deadenylation-dependent manner

doi: 10.1080/15476286.2022.2071026

Figure Lengend Snippet: Proposed model of CCR4-NOT/BRF1 mediated decay of Per2 mRNA.

Article Snippet: Antibodies against the following were used: Cnot1, Cnot3, Cnot6, Cnot6l, Cnot7, and Cnot8 (mouse monoclonal antibodies; generated by Bio Matrix Research and Research Center for Advanced Science and Technology, The University of Tokyo), BRF1/2 (#2119; Cell Signalling Technology), KSRP (A302-021A; Bethyl Laboratories), α-tubulin (#T9026; Sigma), PER2 (PER21-A, Alpha diagnostic) for mouse tissue, PER2 (STJ115134, St. John’s Laboratory) for HEK293T cells, BMAL1 (A302-616A; Bethyl Laboratories), 4E-BP1 (#9644S, Cell Signalling Technology) and GAPDH (#2118; Cell Signalling Technology).

Techniques:

a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of GluA1 S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.

Journal: Nature Communications

Article Title: Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation

doi: 10.1038/s41467-019-13554-y

Figure Lengend Snippet: a Western blotting of the synaptosomal fraction from dorsal hippocampus of dnBMAL1 mice show significant reduction of phosphorylation level of GluA1 S845 (pS845). The graph represents fold changes compared to pS845 levels in WT. b , c Experimental designs are illustrated at the top of each panel. Memory retrieval is impaired at ZT10 in GluA1 S845A knockin (S845A) mice in b social recognition task (recognition index) and c contextual fear memory. dn dnBMAL1. All values are mean ± SEM. Individual data points are displayed as dots. * p < 0.05 as determined by one-way ANOVA with post hoc test. The results of the statistical analyses are presented in Supplementary Table . Source data are provided as a source data file.

Article Snippet: Western blotting was performed using a rabbit polyclonal anti-GluA1 antibody (AB1504, 1:1000; Merck Millipore), anti-GluA1 phospho-Ser845 antibody (AB5849, 1:1000; Merck Millipore), anti-BMAL1 antibody (NB100-2288, 1:1000; Novus Biologicals, Littleton, CO, USA), anti-Per2 antibody (PER21-A, 1:1000; Alpha Diagnostic International, San Antonio, TX, USA), anti-Dbp antibody (PM079, 1:1000; MBL), or mouse monoclonal anti-β-Actin antibody (A1978, 1:10,000; Sigma-Aldrich) , .

Techniques: Western Blot, Knock-In

Both WT and dnBMAL1 mice show a time-of-day retrieval profile. However, dnBMAL1 mice show worse retrieval performance at any time compared to WT mice; dnBMAL1 mice show impairments in retrieval of weak and strong memories at ZT4 and ZT10, respectively, compared to WT mice. Phosphorylation of GluA1 at S845 is required for maintenance of retrieval performance.

Journal: Nature Communications

Article Title: Hippocampal clock regulates memory retrieval via Dopamine and PKA-induced GluA1 phosphorylation

doi: 10.1038/s41467-019-13554-y

Figure Lengend Snippet: Both WT and dnBMAL1 mice show a time-of-day retrieval profile. However, dnBMAL1 mice show worse retrieval performance at any time compared to WT mice; dnBMAL1 mice show impairments in retrieval of weak and strong memories at ZT4 and ZT10, respectively, compared to WT mice. Phosphorylation of GluA1 at S845 is required for maintenance of retrieval performance.

Article Snippet: Western blotting was performed using a rabbit polyclonal anti-GluA1 antibody (AB1504, 1:1000; Merck Millipore), anti-GluA1 phospho-Ser845 antibody (AB5849, 1:1000; Merck Millipore), anti-BMAL1 antibody (NB100-2288, 1:1000; Novus Biologicals, Littleton, CO, USA), anti-Per2 antibody (PER21-A, 1:1000; Alpha Diagnostic International, San Antonio, TX, USA), anti-Dbp antibody (PM079, 1:1000; MBL), or mouse monoclonal anti-β-Actin antibody (A1978, 1:10,000; Sigma-Aldrich) , .

Techniques: